Analysis Note

ControlMouse brain tissue lysate

Application

Research CategoryNeuroscience

Detect Synaptotagmin using this mouse monoclonal antibody, Anti-Synaptotagmin-12 Antibody, clone N277/7 validated for use in western blotting & IHC.

Research Sub CategoryDevelopmental Neuroscience

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Synpatotagmin-12 in mouse brain and rat cerebellum tissues.

Western Blotting Analysis: 1 µg/mL from a representative lot detected Synaptotagmin-12 in 10 µg of human brain tissue lysate.

Western Blotting Analysis: A representative lot detected Synaptotagmin-12 in COS cell lysates transfected with GFP-tagged Synaptotagmin-12, and not in COS cell lysates transiently transfected with other GFP-tagged Synaptotagmins or with untagged Kv2.1 plasmid (Prof. J. Trimmer, University of California, Davis.). Note that GFP tagged proteins run higher than the expected molecular weight of the target protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Synaptotagmin-12 is also called Synaptotagmin XII (SytXII).Synaptotagmin-12 is a member of the synaptotagmin family of transmembrane transporters. Synaptotagmin-12 is thought to be involved in Ca2+-dependent exocytosis of secretory vesicles. Synaptotagmin-12 may also act as Ca2+ sensors as part of the vesicular trafficking and exocytosis process.

Immunogen

Recombinant protein corresponding to mouse Synaptotagmin-12.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Synpatotagmin-12 in 10 µg of mouse brain tissue lysate.

Specificity

This antibody demonstrates specificity for Synaptotagmin-12, and does not cross react with 11 other Synaptotagmins.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~47 kDa observed.